Nondestructive FTIR monitoring of leaf senescence and elicitin-induced changes in plant leaves.

摘要: The applicability of the FTIR attenuated total reflectance technique for in situ monitoring plant physiological processes such as leaf senescence and aging has been examined. Difference spectra obtained by subtracting spectrum young from that older one revealed positive bands at 1650–1500 cm−1, indicating a higher relative concentration phenolics leaves both black cherry sweet pepper bush leaves. Prolonged stress tobacco exhibited progressive time-dependent increase absorbance around 3475 corresponding to hydroxyl functional groups. Absorption changes were also observed between 1650 1500 which are likely correspond phenolics. characteristic resulting induced detected response treatment with recombinant α-elicitin, cinnamomin. This allowed first quantification biological activity elicitin using spectroscopic method. We suggest spectroscopy provides important information about events occurring tissue vivo, it could be useful characterization responsiveness fungal toxins elicitins. © 2003 Wiley Periodicals, Inc. Biopolymers (Biospectroscopy) 72: 79–85,