Innovative Approach for Improvement of an Antibiotic-Overproducing Industrial Strain of Streptomyces albus

摘要: Working with a Streptomyces albus strain that had previously been bred to produce industrial amounts (10 mg/ml) of salinomycin, we demonstrated the efficacy introducing drug resistance-producing mutations for further improvement. Mutants enhanced salinomycin production were detected at high incidence (7 12%) among spontaneous isolates resistant streptomycin (Strr), gentamicin, or rifampin (Rifr). Finally, successfully improvement productivity by 2.3-fold triple mutation. The Strr mutant was shown have point mutation within rpsL gene (encoding ribosomal protein S12). Likewise, Rifr possessed in rpoB RNA polymerase β subunit). Increased (containing K88R S12 protein) may be result an aberrant synthesis mechanism. This aberration manifest itself as translation activity stationary-phase cells, observed poly(U)-directed cell-free system. ribosome characterized increased 70S complex stability low Mg2+ concentrations. We conclude this ability mutant, which is complex, responsible remarkable enhancement obtained.