Transcriptome of the Female Synganglion of the Black-Legged Tick Ixodes scapularis (Acari: Ixodidae) with Comparison between Illumina and 454 Systems

摘要: Illumina and 454 pyrosequencing were used to characterize genes from the synganglion of female Ixodes scapularis. GO term searching success for biological processes was similar samples sequenced by both methods. However, molecular processes, it more successful than samples. Functional assignments transcripts predicting neuropeptides, neuropeptide receptors, neurotransmitter receptors other interest done, supported strong e-values (<−6), high consensus sequence alignments. Transcripts 15 putative prepropeptides ((allatostatin, allatotropin, bursicon α, corticotropin releasing factor (CRF), CRF-binding protein, eclosion hormone, FMRFamide, glycoprotein A, insulin-like peptide, ion transport myoinhibitory inotocin ( = neurophysin-oxytocin), Neuropeptide F, sulfakinin SIFamide)) 14 neuropeptides (allatostatin, calcitonin, cardioacceleratory corazonin, CRF, gonadotropin-releasing hormone/AKH-like, proctolin, pyrokinin, SIFamide, tachykinin) are reported. Similar Dermacentor variabilis, we found matching pro-protein convertase, essential converting hormones their mature form. Additionally, 6 neurotransmitter/neuromodulator (acetylcholine, GABA, dopamine, glutamate, octopamine serotonin) 3 transporters (GABA transporter, noradrenalin-norepinephrine transporter Na+-neurotransmitter/symporter) described. Further, pheromone odorant receptor, gustatory novel GPCR messages, ecdysone nuclear JH esterase binding steroidogenic activating chitin synthase, chitinase, interest. Also spermatogenesis-associated major sperm spermidine oxidase not normally expressed in CNS invertebrates. The diversity messages important identified this study offers a valuable resource useful understanding how tick regulates physiological functions.