Spatiotemporal regulation of T cell costimulation by TCR-CD28 microclusters and protein kinase C theta translocation.

摘要: Summary T cell activation is mediated by microclusters (MCs) containing T cell receptors (TCRs), kinases, and adaptors. Although TCR MCs translocate to form a central supramolecular cluster (cSMAC) of the immunological synapse at interface an antigen-presenting cell, role MC translocation in signaling remains unclear. Here, we found that accumulation cSMAC was important for costimulation. Costimulatory receptor CD28 initially recruited coordinately with MCs, its signals were through assembly kinase PKCθ. The accompanied segregation from TCR, which resulted both PKCθ spatially unique subregion cSMAC. Thus, costimulation generation costimulatory compartment via dynamic regulation translocation.