Sequence verification of synthetic DNA by assembly of sequencing reads.

摘要: Gene synthesis attempts to assemble user-defined DNA sequences with base-level precision. Verifying the of construction intermediates and final product a gene project is critical part workflow, yet one that has received least attention. Sequence validation equally important for other kinds curated clone collections. Ensuring physical sequence matches its published common quality control step performed at once over course research project. GenoREAD web-based application breaks verification process into two steps: assembly sequencing reads alignment resulting contig reference sequence. can determine if Its sophisticated reporting features help identify troubleshoot problems arise during process. been experimentally validated on thousands gene-sized constructs from an ORFeome project, longer including whole plasmids synthetic chromosomes. Comparing results those manual analysis data demonstrates tends be conservative in diagnostic. available www.genoread.org.