作者: A. E. Melchinger , L. Kuntze , R. K. Gumber , T. Lübberstedt , E. Fuchs
关键词: Germplasm 、 Genotype 、 Genetic marker 、 Backcrossing 、 Biology 、 Sugarcane mosaic virus 、 Restriction fragment length polymorphism 、 Gene mapping 、 Plant disease resistance 、 Genetics
摘要: Sugarcane mosaic virus (SCMV) causes considerable damage to maize (Zea mays L.) in Europe. The objective of the present study was determine genetic basis resistance SCMV European germplasm and compare it with that U.S. inbred Pa405. Three resistant inbreds D21, D32, FAP1360A were crossed four susceptible F7, KW1292, D408, D145 produce F2 populations three backcrosses parent. Screening for parental segregating generations done two field trials as well under greenhouse conditions. RFLP markers umc85, bnl6.29, umc10, umc44, SSR marker phi075 used or F3 lines locate gene(s) genome. Segregation backcross fitted different gene models depending on environmental conditions genotype In tests, seems be controlled by genes. Under conditions, susceptibility D32 appears governed one dominant recessive gene. Allelism tests indicated presence a common (denoted Scm1) all Marker analyses mapped genes: Scm1 chromosome 6S Scm2 3.