Recombinant amyloid beta-peptide production by coexpression with an affibody ligand.
作者： Bertil Macao , Wolfgang Hoyer , Anders Sandberg , Ann-Christin Brorsson , Christopher M Dobson
关键词: Amyloid beta 、 Pathogenesis 、 Neurotoxicity 、 Amyloid 、 P3 peptide 、 Biochemistry 、 Biology 、 Recombinant DNA 、 Ligand 、 Peptide
摘要: Background Oligomeric and fibrillar aggregates of the amyloid β-peptide (Aβ) have been implicated in pathogenesis Alzheimer's disease (AD). The characterization Aβ assemblies is essential for elucidation mechanisms neurotoxicity, but requires large quantities pure peptide. Here we describe a novel approach to recombinant production Aβ. method based on coexpression affibody protein ZAβ3, selected affinity ligand derived from Z domain three-helix bundle scaffold. ZAβ3 binds amyloidogenic central C-terminal part with nanomolar consequently inhibits aggregation.
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