Kinetic studies and unstructured models of lymphocyte metabolism in fed-batch culture.

摘要: The growth of two lymphocyte cell lines, a hybridoma line and human cutaneous T lymphoma (HuT78), was studied in fed-batch culture, unstructured models developed. A criteria established to insure that the rate varied by less than specified tolerance throughout culture period. Glutamine serum were growth-limiting nutrients for both lines with half-maximal rates at 0. 53 mM glutamine 55%(v/v) cells 21 1. 5% HuT-78 cells. Over range glucose concentrations from 5. 5 28 mM, specific independent concentration, whereas above inhibited growth. For significantly addition ammonium, although more affected ammonia line. Growth increased presence interleukin-2. Unstructured similar other presented literature. Applications these adoptive immunotherapy are discussed.