Survival of various ERIC-genotypes of Shiga toxin-producing Escherichia coli in well water

作者: Leigh Watterworth , Bruce Rosa , Heidi Schraft , Edward Topp , Kam Tin Leung

DOI: 10.1007/S11270-006-9179-X

关键词: GenotypeMicrobiologyStrain (chemistry)Escherichia coliBacteriaInoculationIntergenic regionEnterobacteriaceaeBiologyShiga-like toxin

摘要: Recently, there has been a surge of interest in understanding the survival Shiga toxin-producing Escherichia coli (STEC) aquatic environments. Fifteen strains STEC were monitored, individually, untreated well water samples incubated at 10 and 22^C for 56 days. The selected from three serogroups (O26, Ol11 O157) represented five distinct ERIC (enterobacterial repetitive intergenic concensus)-genotypes. microcosms prepared triplicate inoculated an initial cell density about 7.0 log CFU/ml water. At 10^C, fell below detection limit 0.8 by day 56. Of ten persisting strains, four showed superior with densities decreasing to average 5 while remaining six moderate levels survival, 3 CFU/ml. 22^C, strain H32 (genotype I) H15 B) persisted 1.1 2.2 days, respectively. other 13 dropped between weeks 8. 15 demonstrated highly variable no correlation ERIC-genotypes or strains' ability persist samples. Although (O157:H7) significantly longer than H22 natural both survived equally sterile water, indicating that individual vary their compete background microbial populations.

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