Dietary effects on resting metabolic rate in C57BL/6 mice are differentially detected by indirect (O2/CO2 respirometry) and direct calorimetry.

作者: Colin M.L. Burnett , Justin L. Grobe

DOI: 10.1016/J.MOLMET.2014.03.003

关键词: Internal medicineMetabolismBioinformaticsMetabolic rateDirect calorimetryBasal metabolic rateRespirometryEndocrinologyBiologyHigh fat dietC57BL/6

摘要: Abstract Resting metabolic rate (RMR) studies frequently involve genetically-manipulated mice and high fat diets (HFD). We hypothesize that the use of inadequate methods impedes identification novel regulators RMR. This idea was tested by simultaneously measuring RMR direct calorimetry respirometry in C57BL/6J fed chow, 45% HFD, then returned to chow. Comparing results during chow feeding uncovered an underestimation (0.010 ± 0.001 kcal/h, P

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