Suppression of tumor growth in lung cancer xenograft model mice by poly(sorbitol-co-PEI)-mediated delivery of osteopontin siRNA.

摘要: Small interfering RNA (siRNA)-mediated gene silencing represents a promising strategy for treating diseases such as cancer; however, specific requires an effective delivery system to overcome the instability and low transfection efficiency of siRNAs. To address this issue, polysorbitol-based transporter (PSOT) was prepared by molecular weight branched polyethylenimine (bPEI) crosslinked with sorbitol diacrylate (SDA). Osteopontin (OPN) gene, which is highly associated non-small cell lung cancer (NSCLC) targeted siRNA therapy using targeting OPN (siOPN). Characterization study confirmed that PSOT formed compact complexes siOPN protected against degradation RNase. PSOT/siOPN demonstrated cytotoxicity enhanced in vitro, suggesting carrier may be suitable silencing. In A549 H460 lines, significant at both protein levels. vivo tumor growth suppression, two cell-xenograft mouse models were delivered into mice through intravenous injection. The siOPN-treated groups significantly reduced expression levels well suppression volume weight. Taken together, present novel therapeutic treatment.