Molecular epidemiological and serological studies of bovine leukemia virus (BLV) infection in Thailand cattle

摘要: BLV is the etiological agent of enzootic bovine leucosis. has negative effects on animal health and causes economic losses worldwide. However, epidemiological studies are relatively unknown in many parts Asian countries. Thus, this study sought to explore infections cattle Thailand determine extent geographic distribution measure its prevalence rates. For study, 744 from 11 farms 9 provinces were screened 2013 2014 by ELISA nested PCR. Of those cattle, 41 BLVs genetically characterized using 188 gp51 env gene sequences available GenBank. The was high, ranging 5.3% 87.8%, as determined PCR 11.0% 100% ELISA, according geographical region. Phylogenetic analysis showed that belonged genotypes 1 6 a new genotype 10, which sporadically observed across with 31.7%, 19.5%, 48.8%, respectively. A significant number amino acid substitutions also found sequences, unique changes 10 have not been reported previously. Briefly, majority confined CD4+/CD8+ T-cell epitopes, neutralizing domains, E-D-A epitopes. Those observations indicate prevalent dynamic, high level genetic diversity. This implies long-term infection populations movement infected cattle. In sum, suggests intensive surveillance effective prevention strategies required control continuous BLVs.