A histidine-kinase cheA gene of Pseudomonas pseudoalcaligens KF707 not only has a key role in chemotaxis but also affects biofilm formation and cell metabolism.
作者： V. Tremaroli , S. Fedi , S. Tamburini , C. Viti , E. Tatti
DOI: 10.1080/08927014.2010.537099
关键词: Mutant 、 Pseudomonas pseudoalcaligenes 、 Methyl-accepting chemotaxis protein 、 Pseudomonas 、 Biology 、 Biofilm 、 Biochemistry 、 Histidine kinase 、 Periplasmic Binding Proteins 、 Chemotaxis
摘要: A histidine-kinase cheA gene in Pseudomonas pseudoalcaligenes KF707 plays a central role the regulation of metabolic responses as well chemotaxis. Non-chemotactic mutants harboring insertions into were screened for their ability to form biofilms Calgary biofilm device. Notably, ≥95% decrease number cells attached polystyrene surface was observed compared wild-type phenotype. The mature restored levels, providing functional copies mutants. In addition, phenotype micro-arrays and proteomic analyses revealed that several basic activities few periplasmic binding proteins mutant differed those cells. These results are interpreted evidence strong integration between chemotactic pathways process development by P. KF707.
sci-hub.se 参考文章(62)
William C. Ghiorse, John T. Wilson, Microbial ecology of the terrestrial subsurface. Advances in Applied Microbiology. ,vol. 33, pp. 107- 172 ,(1988) , 10.1016/S0065-2164(08)70206-5
Davide Zannoni, Francesca Borsetti, Joe J. Harrison, Raymond J. Turner, The bacterial response to the chalcogen metalloids Se and Te. Advances in Microbial Physiology. ,vol. 53, pp. 1- 71 ,(2007) , 10.1016/S0065-2911(07)53001-8
L. W. Enquist, Thomas J. Silhavy, Michael L. Berman, Experiments With Gene Fusions ,(1984)
Heinrich Lonsdorf, Carsten Strompl, A. Mark Osborn, Antonio Bennasar, Edward R.B. Moore, Wolf-Rainer Abraham, Kenneth N. Timmis, [26] Approach to analyze interactions of microorganisms, hydrophobic substrates, and soil colloids leading to formation of composite biofilms, and to study initial events in microbiogeological processes Methods in Enzymology. ,vol. 336, pp. 317- IN14 ,(2001) , 10.1016/S0076-6879(01)36598-9
V de Lorenzo, M Herrero, U Jakubzik, K N Timmis, Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria. Journal of Bacteriology. ,vol. 172, pp. 6568- 6572 ,(1990) , 10.1128/JB.172.11.6568-6572.1990
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
U B Priefer, R Simon, A Pühler, Extension of the host range of Escherichia coli vectors by incorporation of RSF1010 replication and mobilization functions. Journal of Bacteriology. ,vol. 163, pp. 324- 330 ,(1985) , 10.1128/JB.163.1.324-330.1985
Valentina Tremaroli, Caterina Vacchi Suzzi, Stefano Fedi, Howard Ceri, Davide Zannoni, Raymond J. Turner, Tolerance of Pseudomonas pseudoalcaligenes KF707 to metals, polychlorobiphenyls and chlorobenzoates: effects on chemotaxis‐, biofilm‐ and planktonic‐grown cells FEMS Microbiology Ecology. ,vol. 74, pp. 291- 301 ,(2010) , 10.1111/J.1574-6941.2010.00965.X
Joe J Harrison, Raymond J Turner, Howard Ceri, High-throughput metal susceptibility testing of microbial biofilms. BMC Microbiology. ,vol. 5, pp. 53- 53 ,(2005) , 10.1186/1471-2180-5-53
K Furukawa, T Miyazaki, Cloning of a gene cluster encoding biphenyl and chlorobiphenyl degradation in Pseudomonas pseudoalcaligenes. Journal of Bacteriology. ,vol. 166, pp. 392- 398 ,(1986) , 10.1128/JB.166.2.392-398.1986