Properties of Thirteen Viruses and Virus Variants Obtained from Eight Isolates of the Wheat Take-All Fungus, Gaeumannomyces graminis var. tritici.

作者: K. W. Buck , M. R. Almond , J. J. P. McFadden , M. A. Romanos , C. J. Rawlinson

DOI: 10.1099/0022-1317-53-2-235

关键词: VirologyTake-allFungusRNA virusGaeumannomyces graminis var. triticiVirusCapsidBiologyMicrobiologyGroup iiRNA silencing

摘要: Summary The properties of polyhedral double-stranded (ds)RNA virus particles obtained from eight isolates the wheat take-all fungus, Gaeumannomyces graminis var. tritici, have been investigated. Thirteen viruses and variants were distinguished these classified into three groups on basis serological physical particles; in a group related serologically to other members same group, but unrelated groups. Group I had diam. 35 nm sedimenting at 109 126S; capsid contained one polypeptide species, mol. wt. 54 × 103 60 dsRNA consisted two four components, 1.0 106 1·3 106. II 133 140S; 68 73 components with 1·39 1·60 III 40 159 163S; 78 103, 83 87 3·2 4·3 106. Five fungal only single viruses. However, isolate II, each I, III. Investigation ten conidial derived latter has shown that all present cell.

参考文章(12)
Alice S. Huang, David Baltimore, Defective Interfering Animal Viruses Springer, Boston, MA. pp. 73- 116 ,(1977) , 10.1007/978-1-4684-0832-4_2
Michael Hollings, Mycoviruses: Viruses that Infect Fungi Advances in Virus Research. ,vol. 22, pp. 1- 53 ,(1978) , 10.1016/S0065-3527(08)60771-X
C.H.W. Hirs, [6] Determination of cystine as cysteic acid Methods in Enzymology. ,vol. 11, pp. 59- 62 ,(1967) , 10.1016/S0076-6879(67)11008-2
J Bruenn, W Kane, Relatedness of the double-stranded RNAs present in yeast virus-like particles. Journal of Virology. ,vol. 26, pp. 762- 772 ,(1978) , 10.1128/JVI.26.3.762-772.1978
J.S. Semancik, A.K. Vidaver, J.L. Van Etten, Characterization of a segmented double-helical RNA from bacteriophage φ6 Journal of Molecular Biology. ,vol. 78, pp. 617- 625 ,(1973) , 10.1016/0022-2836(73)90283-0
Linda J. Frick, Richard M. Lister, Serotype variability in virus-like particles from Gaeumannomyces graminis☆ Virology. ,vol. 85, pp. 504- 517 ,(1978) , 10.1016/0042-6822(78)90457-9
K. W. Buck, G. F. Kempson-Jones, Capsid Polypeptides of Two Viruses Isolated from Penicillium stoloniferum Journal of General Virology. ,vol. 22, pp. 441- 445 ,(1974) , 10.1099/0022-1317-22-3-441
K. W. Buck, G. Ratti, Biophysical and Biochemical Properties of Two Viruses Isolated from Aspergillus foetidus Journal of General Virology. ,vol. 27, pp. 211- 224 ,(1975) , 10.1099/0022-1317-27-2-211