Chronic ethanol ingestion by mice increases expression of CD80 and CD86 by activated macrophages.

摘要: Results from previous studies our laboratory have shown that T cells obtained the spleens of C57BL/6 mice consumed ethanol chronically increased expression activation markers and second signal-independent production interferon-gamma (IFN-gamma). We now report in vitro-activated CD11b(+) splenocytes BALB/c express levels cell co-stimulatory molecules CD80 CD86. encompass at least two populations: CD11b(+)Gr.1(-) population, which is primarily monocytes-macrophages, a smaller CD11b(+)Gr.1(+) myelocytic-monocytic series contains precursors both macrophages neutrophils. Evaluation cultures purified cells, chronically, incubated overnight, showed up-regulation CD86 on Gr.1(-) mouse splenic macrophages. functional demonstrated were exposed to secrete higher levels, comparison with secreted by control animals, nitric oxide several proinflammatory cytokines after stimulation oligodeoxynucleotide (ODN) CpG 1826. These findings indicate are some way sensitized activating stimuli chronic exposure vivo. Such may contribute systemic immunodysregulation, including T-cell activation, providing abnormal signals or through excessive release cytokines, such as interleukin (IL)-6 IL-12.