Etiology of MNU-induced melanomas in Xiphophorus hybrids
作者： Jennifer J. Rahn , David Trono , Irma Gimenez-Conti , Andrew P. Butler , Rodney S. Nairn
DOI: 10.1016/J.CBPC.2008.07.006
关键词: Hybrid 、 Candidate gene 、 Backcrossing 、 Xiphophorus 、 Gene 、 Melanoma 、 Locus (genetics) 、 Biology 、 Nitrosourea 、 Genetics
摘要: Abstract Genetic hybrids of the genus Xiphophorus have historically been useful models for study genetic aspects tumor formation. In most studied model, two-gene loci, XMRK and DIFF , are implicated as critical both to UV-induced spontaneous melanoma formation in BC 1 crosses between X. maculatus helleri with recurrent backcross parent. addition UV, direct-acting carcinogen N -methyl- -nitrosourea (MNU) has used induce tumors from several cross types. present study, we address hypothesis that excess melanomas MNU-treated may generated by direct mutation CDKN2AB a candidate gene . MNU treatment F hybrid fish significantly increased incidence at 6 months; however, no association was found MNU-induced zygosity tumor-suppressor hybrids, consistent previously reported results. Sequence analysis locus heterozygous individuals (both hybrids) did not reveal any mutations caused MNU, suggesting mechanism this model does involve but result other genes.
elsevier.com
researchgate.net 
sci-hub.se 参考文章(26)
A. Schartl, M. Pagany, M. Engler, M. Schartl, Analysis of genetic factors and molecular mechanisms in the development of hereditary and carcinogen-induced tumors of Xiphophorus. Recent results in cancer research. ,vol. 143, pp. 225- 235 ,(1997) , 10.1007/978-3-642-60393-8_15
Ling Liu, David Dilworth, Luzhang Gao, Jose Monzon, Ann Summers, Norman Lassam, David Hogg, Mutation of the CDKN2A 5' UTR creates an aberrant initiation codon and predisposes to melanoma Nature Genetics. ,vol. 21, pp. 128- 132 ,(1999) , 10.1038/5082
Manfred Schartl, Homology of melanoma-inducing loci in the genus Xiphophorus. Genetics. ,vol. 126, pp. 1083- 1091 ,(1990) , 10.1093/GENETICS/126.4.1083
A. Gomez, Identification of a Second egfr Gene in Xiphophorus Uncovers an Expansion of the Epidermal Growth Factor Receptor Family in Fish Molecular Biology and Evolution. ,vol. 21, pp. 266- 275 ,(2003) , 10.1093/MOLBEV/MSH017
Saraswati Sukumar, Barbara Armstrong, Joost P. Bruyntjes, Irwin Leav, Maarten C. Bosland, Frequent activation of the Ki-ras oncogene at codon 12 in N-methyl-N-nitrosourea-induced rat prostate adenocarcinomas and neurogenic sarcomas. Molecular Carcinogenesis. ,vol. 4, pp. 362- 368 ,(1991) , 10.1002/MC.2940040507
Steven Kazianis, Irma Gimenez-Conti, David Trono, Anthony Pedroza, Lori B. Chovanec, Donald C. Morizot, Rodney S. Nairn, Ronald B. Walter, Genetic Analysis of Neoplasia Induced by N-Nitroso-N-methylurea in Xiphophorus Hybrid Fish Marine Biotechnology. ,vol. 3, ,(2001) , 10.1007/S10126001-0025-2
D Adam, N Dimitrijevic, M Schartl, Tumor suppression in Xiphophorus by an accidentally acquired promoter Science. ,vol. 259, pp. 816- 819 ,(1993) , 10.1126/SCIENCE.8430335
Cara L. Benjamin, Vladislava O. Melnikova, Honnavara N. Ananthaswamy, Models and mechanisms in malignant melanoma. Molecular Carcinogenesis. ,vol. 46, pp. 671- 678 ,(2007) , 10.1002/MC.20353
Juergen R. Vielkind, Klaus D. Kallman, Donald C. Morizot, Genetics of Melanomas in Xiphophorus Fishes Journal of Aquatic Animal Health. ,vol. 1, pp. 69- 77 ,(1989) , 10.1577/1548-8667(1989)001<0069:GOMIXF>2.3.CO;2
Mark Harland, Elizabeth A. Holland, Paola Ghiorzo, Michela Mantelli, Giovanna Bianchi-Scarr�, Alisa M. Goldstein, Margaret A. Tucker, Bruce A.J. Ponder, Graham J. Mann, D. Timothy Bishop, Julia Newton Bishop, Mutation screening of the CDKN2A promoter in melanoma families. Genes, Chromosomes and Cancer. ,vol. 28, pp. 45- 57 ,(2000) , 10.1002/(SICI)1098-2264(200005)28:1<45::AID-GCC6>3.0.CO;2-F