Effects of Various Selective Phosphodiesterase Inhibitors on Muscle Contractility in Guinea Pig Ileal Longitudinal Smooth Muscle.
作者： Takeharu Kaneda , Kazumasa Shimizu , Shinjiro Nakajyo , Norimoto Urakawa
DOI: 10.1254/JJP.75.77
关键词: Contractility 、 Phosphodiesterase inhibitor 、 Internal medicine 、 IBMX 、 Muscle contraction 、 Muscle tension 、 Zaprinast 、 Endocrinology 、 Chemistry 、 Phosphodiesterase 、 Phosphodiesterase 3 、 Pharmacology
摘要: The effects of various selective phosphodiesterase (PDE) inhibitors on muscle contractility in guinea pig ileal longitudinal smooth were investigated. 1) 3-Isobutyl-l-methyl xanthine (IBMX) or zaprinast markedly inhibited the high K+ carbachol (CCh)-induced contraction and increased cGMP content strip a concentration-dependent manner. However, these agents only slightly cAMP content. Milrinone Ro20-1724 also CCh-induced content, but did not increase cGMP. 2) In fura2-loaded muscle, IBMX both contractions intracellular Ca2+ ([Ca2+]i) level induced by CCh, although inhibitory effect [Ca2+]i was smaller than that tension. 3) α-toxin-permeabilized muscles, cGMP, significantly Ca2+induced contraction. These results suggest inhibit muscles mainly through an mechanism is involved decreases sensitivity contractile elements to Ca2+.
sci-hub.se 参考文章(24)
T Kitazawa, S Kobayashi, K Horiuti, A V Somlyo, A P Somlyo, Receptor-coupled, permeabilized smooth muscle. Role of the phosphatidylinositol cascade, G-proteins, and modulation of the contractile response to Ca2+. Journal of Biological Chemistry. ,vol. 264, pp. 5339- 5342 ,(1989) , 10.1016/S0021-9258(18)83550-5
J A Beavo, M Conti, R J Heaslip, Multiple cyclic nucleotide phosphodiesterases. Molecular Pharmacology. ,vol. 46, pp. 399- 405 ,(1994)
D H Maurice, R J Haslam, Molecular basis of the synergistic inhibition of platelet function by nitrovasodilators and activators of adenylate cyclase: inhibition of cyclic AMP breakdown by cyclic GMP. Molecular Pharmacology. ,vol. 37, pp. 671- 681 ,(1990)
R F Kauffman, K W Schenck, V G Crowe, M L Cohen, B G Utterback, In vitro vascular relaxation by new inotropic agents: relationship to phosphodiesterase inhibition and cyclic nucleotides. Journal of Pharmacology and Experimental Therapeutics. ,vol. 242, pp. 864- 872 ,(1987)
R M Rapoport, Cyclic guanosine monophosphate inhibition of contraction may be mediated through inhibition of phosphatidylinositol hydrolysis in rat aorta. Circulation Research. ,vol. 58, pp. 407- 410 ,(1986) , 10.1161/01.RES.58.3.407
Sheu-Meei Yu, Zhi-Jiao Cheng, Sheng-Chu Kuo, Endothelium-dependent relaxation of rat aorta by butein, a novel cyclic AMP-specific phosphodiesterase inhibitor. European Journal of Pharmacology. ,vol. 280, pp. 69- 77 ,(1995) , 10.1016/0014-2999(95)00190-V
David J. Newman, Donald F. Colella, Charles B. Spainhour, Edward G. Brann, Bogdan Zabko-Potapovich, Joe R. Wardell, cAMP-phosphodiesterase inhibitors and tracheal smooth muscle relaxation. Biochemical Pharmacology. ,vol. 27, pp. 729- 732 ,(1978) , 10.1016/0006-2952(78)90511-7
P Collins, T M Griffith, A H Henderson, M J Lewis, Endothelium-derived relaxing factor alters calcium fluxes in rabbit aorta: a cyclic guanosine monophosphate-mediated effect. The Journal of Physiology. ,vol. 381, pp. 427- 437 ,(1986) , 10.1113/JPHYSIOL.1986.SP016336
W. D. M. Paton, M. Aboo Zar, The origin of acetylcholine released from guinea‐pig intestine and longitudinal muscle strips The Journal of Physiology. ,vol. 194, pp. 13- 33 ,(1968) , 10.1113/JPHYSIOL.1968.SP008392
Junji Nishimura, Cornelis van Breemen, Direct regulation of smooth muscle contractile elements by second messengers Biochemical and Biophysical Research Communications. ,vol. 163, pp. 929- 935 ,(1989) , 10.1016/0006-291X(89)92311-5