Identification of Benzo(a)pyrene Metabolites by Gas Chromatograph-Mass Spectrometer

摘要: A gas chromatograph-mass spectrometer was used in an attempt to achieve rapid separation and accurate identification of benzo(a)pyrene its synthesized derivatives. All derivatives, after being trimethylsilylated, were developed on Dexsil-300 OV-1 columns. The seven diols four stereoisomeric 7,8,9,10-tetraols studied separated successfully. the 12 phenols unsatisfactory; 8- 11-isomers appeared separately but other 10 isomers made 3 peaks column. Among five derivatives reported be present animal tissues, 6-, 7-, 9-phenols separated, 1- 3-phenols fused Quinones converted related dihydroxyl under silylation. chromatographic six successful column, 6,12- 7,10-isomers remained a single peak. two diol-epoxides unstable silylation therefore detected by their breakdown products, 7,8,9-trihydroxy-7,8-dihydrobenzo( )pyrene tetraols. Data are listed mass spectra retention times all benzo( studied.