The repair of low dose UV light-induced damage to human skin DNA in condition of trace amount Mg 2+

摘要: Ultraviolet light-induced damage to human skin DNA was widely investigated. The primary mechanism of this damage contributed form cyclobutane pyrimidine dimmers (CPDs). Although the distribution UV CPDs within a defined sequence is similar, in cellular environment which shields nuclear higher than that organism apparent dose. So we use low UVB light as main study agent. Low dose UV-irradiated HDF-a cells (Human Dermal Fibroblasts-adult cells) weaker than epidermic cells were cultured with DMEM at different trace amount Mg 2+ (0mmol/L , 0.1mmol/L 0.2mmol/L, 0.4mmol/L, 0.8mmol/L, 1.2mmol/L) free-serum and the repair strands injured observed. Treat these strand breaks detection, photoproducts detection and photoproducts detection. Then quantitate role trace UV light-induced skin. experiment results indicated that have capability resistance to UV-radiation certain extent. And can regulate UV-induced relative vitality. It can offer rationale data relieve disease.