A universal label-free biosensing platform based on opto-fluidic ring resonators

摘要: Rapid and accurate detection of biomolecules is important for medical diagnosis, pharmaceuticals, homeland security, food quality control, environmental protection. A simple, low cost highly sensitive label-free optical biosensor based on opto-fluidic ring resonator (OFRR) has been developed that naturally integrates microfluidics with resonators. The OFRR employs a piece fused silica capillary with diameter around 100 micrometers. circular cross section the capillary forms resonator and light repeatedly travels along circumference in form whispering gallery modes (WGMs) through total internal reflection. When wall as thin couple micrometers (< 4 μm), an evanescent field WGMs exists at inner surface interacts sample when it flows OFRR. In order to detect target molecules high specificity, inner surface functionalized receptors, such antibodies, peptide-displayed bacteriophage or oligonucleotide DNA probes. WGM spectral position shifts when bind OFRR inner change local refractive index, which provides quantitative kinetic information about biomolecule interaction near surface. successfully demonstrated various types biomoelcuels. Here, we will first introduce basic operation principle sensor then application examples of proteins, disease biomarkers, virus, molecules, cells sensitivities be presented.