Effects of mitotane on gene expression in the adrenocortical cell line NCI-H295R: a microarray study
作者： Adrienn Zsippai , Diana Rita Szabó , Zsófia Tömböl , Peter M Szabó , Katalin Éder
DOI: 10.2217/PGS.12.116
关键词: Mitotane 、 Gene expression 、 Hormone 、 HSD3B1 、 Steroid biosynthesis 、 Endocrinology 、 HSD3B2 、 Microarray 、 Cancer research 、 Internal medicine 、 Viability assay 、 Biology
摘要: Aim: The adrenolytic agent mitotane is widely used in the treatment of adrenocortical cancer; however, its mechanism action poorly elucidated. We have studied mitotane-induced mRNA expression changes NCI-H295R cancer cell line. Materials & methods: Cell viability and hormone assays were to select optimal concentration effectively inhibiting secretion without affecting viability. RNA isolated from cultures treated for 48 72 h was subjected Agilent 4×44K microarray platforms. Microarray results validated by quantitative reverse-transcription PCR. Results: Altogether, 117 significantly differentially expressed genes detected at (p < 0.05) mitotane-treated samples relative controls. Three underexpressed involved steroid biosynthesis (HSD3B1, HSD3B2 CYP21A2) four overexpressed ( GDF15, ALDH1L2, TRIB3 SERPINE2) been validated. Conclusion: Gene-expression might be adrenal inhibition secretion. Original submitted 20 January 2012; Revision 17 May 2012
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