Two-step isolation of hemicellulose from oil palm empty fruit bunch fibers and its use in production of xylooligosaccharide prebiotic

摘要: Abstract Hemicellulose was recovered from oil palm empty fruit bunch (EFB) fiber and used for producing xylooligosaccharides (XOS) prebiotics. EFB fibers were treated sequentially with peracetic acid (PA) alkaline peroxide (AP). The PA treatment removed ∼50 % lignin. subsequent AP the hemicellulose. optimized using Taguchi experiment design L16 orthogonal array consisting of 5 factors (NaOH concentration, H2O2 MgSO4 incubation temperature, time) 4 levels. Data 16 experimental runs showed that main effect on percentage hemicellulose recovery in order: NaOH > MgSO4 > temperature > time H2O2. optimal conditions isolation were: 15 w/v NaOH reaction system (3 g NaOH/g delignified EFB), 1% (0.2 g MgSO4/g 45 °C temperature a 3 h time. PA-AP treatments solubilized nearly 53 original dry mass EFB. enriched yield 18.4 by weight. Acid hydrolysis released produced xylose (44.1 %), glucose (17.6 arabinose (2.2 galactose mannose (4.4 %). Thermomyces lenuginosus xylanase (294.4 U L−1) to produce XOS at 55 °C 12-h reaction. assessed as substrates two probiotic bacteria found human gastrointestinal tract. Lactobacillus plantarum WU-P19 utilized better than Bifidobacterium bifidum TISTR 2129. Coculture shown mixed fatty acids.