Effects of an immunomodulatory feed additive on the development of mastitis in a mouse infection model using four bovine-origin isolates.

摘要: The goal of this study was to examine the ability a commercially available feed additive (OmniGen-AF) reduce mammary infections caused by single strain mastitic pathogens (Streptococcus uberis, Escherichia coli, Staphylococcus aureus and Klebsiella pneumoniae) effects on markers immunity. Four experiments were completed using murine model bovine mastitis. Infection progression examined Sybr-green- TaqMan-based quantitative PCR assays 16S ribosomal DNA. gland with all rapid (24 48 h) appearance pathogen DNA in tissue. Provision for 2 weeks before infection significantly (P < 0.05) reduced extent accumulation models S. E. coli infection. ineffective reducing K. pneumoniae. We mechanisms action through assessment concentrations myeloperoxidase (MPO), major histocompatibility complex class II (MHC) macrophage inflammatory protein-1α (MIP) messenger RNA (mRNA) examining serum complement C3 concentration. increased MPO MHC mRNAs 0.05). Ability elicit changes gene expression enhanced provision These data imply that response antigen presentation during Value preventing mastitis cattle awaits additional studies further evaluation strains used study.