A novel and simple microcontact printing technique for tacky, soft substrates and/or complex surfaces in soft tissue engineering

摘要: Microcontact printing (μCP) has attracted much interest due to its simplicity and wide range of applications. However, when conventional μCP is applied soft and/or tacky substrates, substrate sagging difficulty in stamp removal cause non-conformance the patterns. Moreover, it almost impossible apply on complex or wavy surfaces. In this study, we developed a novel yet simple trans-print method create efficient micropatterning substrates such as polydimethylsiloxane polyacrylamide gel, also curved surfaces, by introducing polyvinyl alcohol film media. This technique only involves one step cost-effective. Most importantly, versatile have proven various designs more non-flat surfaces using proteins inks. The quality trans-printed pattern was excellent with high reproducibility resolution verified immunostaining. Human mesenchymal stem cells cultured these patterns displayed good conformance printed technique. These results suggest that can be extended potentially generic methodology for other biomolecules, shapes sizes, cells, will useful three-dimensional tissue engineering.