Determination of N-acetylglucosaminyltransferases III, IV and V in normal and hepatoma tissues of rats.
作者： Atsushi Nishikawa , Jianguo Gu , Shigeru Fujii , Naoyuki Taniguchi
DOI: 10.1016/0304-4165(90)90094-D
关键词: Alpha (ethology) 、 Substrate (chemistry) 、 High-performance liquid chromatography 、 Sugar 、 Beta (finance) 、 Small intestine 、 Biochemistry 、 MOPS 、 Enzyme 、 Chemistry
摘要: N-Acetylglucosaminyltransferase III, IV and V activities were assayed in various rat tissues hepatomas using the same fluorescence-labeled sugar chain, GlcNAc beta 1-2Man alpha 1-3-(GlcNAc 1-6)Man 1-4GlcNAc 1-4GlcNAc-2-aminopyridine as a substrate. The N-acetylglucosaminyltransferase III activity toward substrate is highest most including primary hepatoma. A relatively higher for GnT-V found small intestine, serum hepatoma compared to that of GnT-IV. Some kinetic properties these enzymes crude extracts also determined.
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