Inhibitory Effect of Zinc Protoporphyrin IX on Lower Esophageal Sphincter Smooth Muscle Relaxation by Vasoactive Intestinal Polypeptide and Other Receptor Agonists
作者： Ya-Ping Fan , Satish Rattan , Sushanta Chakder
DOI:
关键词: Cyclase 、 Endocrinology 、 Sodium nitroprusside 、 Chemistry 、 G protein 、 Forskolin 、 Bethanechol 、 Smooth muscle contraction 、 Vasoactive intestinal peptide 、 Heme oxygenase 、 Internal medicine
摘要: This study was performed in the opossum lower esophageal sphincter (LES) smooth muscle strips to determine action of heme oxygenase inhibitor zinc protoporphyrin IX (ZnPP IX) on relaxant effect vasoactive intestinal polypeptide and isoproterenol, which are known stimulate adenylate cyclase (AC) via G protein coupling, direct activator AC catalytic subunit forskolin. To investigate cGMP pathway, we examined atrial natriuretic factor activate receptor linked particulate guanylate coupling that sodium nitroprusside [nitric oxide (NO) donor], authentic NO carbon monoxide, intracellular soluble fraction GC. The relaxation caused by nonadrenergic noncholinergic (NANC) nerve stimulation also investigated. ZnPP concentration-dependent attenuation polypeptide, isoproterenol without any forskolin, nitroprusside, CO. Interestingly, had no significant LES NANC contraction bethanechol. From these results, conclude attenuates protein-coupled receptors cyclase. lack nerve-mediated suggests either a role pathway response or an up-regulation NOS leading normal relaxation.
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