Immunoelectron microscopic localization of synaptophysin in a golgi subcompartment of developing hypothalamic neurons

摘要: Abstract Synaptophysin, previously identified as an integral membrane glycoprotein (mol. wt 38,000) characteristic of presynaptic vesicles mature neurons, provides a molecular marker to study the origin, formation and traffic synaptic vesicles. Using monoclonal antibody SY38 against this polypeptide we have localized synaptophysin by immunofluorescence electron microscope immunoperoxidase methods in cultured mouse hypothalamic neurons taken from 16-day-old fetuses which achieve synaptogenesis after 10–12 days vitro. We compared localization perikarya nerve endings function age (2–19 vitro) treatment with nocodazole. microscopy, was already detected neuronal soma at 2 vitro, where initiation neurite development is observed. At level, virtually all boutons varicosities showed extensive labeling 12–13 culture whereas neurites only very few labeled In before synapse (6 vitro), selectively membranes innermost cisternae Golgi zone variable size shape core zone. contrast, formation, barely but strong Treatment (12 nocodazole (10−5 M) resulted conspicuous staining trans-Golgi numerous cytoplasm. Furthermore, accumulation on terminals found. The data suggest that released apparatus vesicular form, glycosylation then transported mechanism requires integrity microtubules.