Saturated fat-induced changes in Sf 60-400 particle composition reduces uptake of LDL by HepG2 cells.
作者： Kim G. Jackson , Vatsala Maitin , David S. Leake , Parveen Yaqoob , Christine M. Williams
DOI: 10.1194/JLR.M500382-JLR200
关键词: Apolipoprotein B-48 、 Endocrinology 、 Saturated fat 、 Biochemistry 、 LDL receptor 、 Internal medicine 、 Postprandial 、 Chemistry 、 Apolipoprotein B 、 Apolipoprotein C-III 、 Polyunsaturated fatty acid 、 Apolipoprotein E
摘要: The ability of human postprandial triacylglycerol-rich lipoproteins (TRLs), isolated after meals enriched in saturated fatty acids (SFAs), n-6 PUFAs, and MUFAs, to inhibit the uptake 125I-labeled LDL by receptor was investigated HepG2 cells. Addition TRLs resulted a dose-dependent inhibition heparin-releasable binding, cell-associated radioactivity, degradation products (P < 0.001). SFA-rich Svedberg flotation rate (Sf) 60-400 significantly greater radioactivity than PUFA-rich particles = 0.016) total compared with PUFA- MUFA-rich 0.02). Normalization apolipoprotein (apo)E but not apoC-III content removed effect meal acid composition, addition an anti-apoE antibody reversed inhibitory on LDL. Real time RT-PCR showed that Sf increased expression genes involved hepatic lipid synthesis 0.05) decreased receptor-related protein 1 MUFAs 0.008). In conclusion, these findings suggest alternative or additional mechanism whereby acute fat ingestion can influence clearance via competitive apoE-dependent effects TRL receptor.
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