Population kinetics of carcinoma cells, capillary endothelial cells, and fibroblasts in a transplanted mouse mammary tumor.

摘要: A combination of histological stains has been used to obtain maximum contrast between capillary endothelial cells, fibroblasts, and parenchymal cells in sections a transplanted C3H mouse mammary tumor. Values kinetic parameters were estimated by thymidine labeling techniques autoradiography for each these three cell populations. The mean index carcinoma was 35%. Mitotic index, grain count per labeled decreased with distance from capillary; there migration regions rapid proliferation near blood vessels slow or non-proliferation necrosis. The duration the cycle phases mitoses experiment, computer methods analyze results. median time 13 hr, growth fraction repeated experiment be about 50%. Therefore, turnover 22 hr. The indices fibroblasts 11.4 9.1%, respectively. These rarely recognized mitosis, their times experiment. results consistent 50 60 hr 70 80 wide spread distribution intermitotic times. The remained quite constant 10 40 after single injection tritiated thymidine; this result implies that are not derived faster-proliferating precursor population. Extension network growing tumor appears depend mainly on division within walls.