A rapid two-step algorithm detects and identifies clinical macrolide and beta-lactam antibiotic resistance in clinical bacterial isolates

摘要: Abstract Purpose Aiming to identify macrolide and beta-lactam resistance in clinical bacterial isolates rapidly accurately, a two-step algorithm was developed based on detection of eight antibiotic genes. Methods Targeting at genes linked ( msrA , ermA ermB ermC ) bla TEM SHV CTX-M-1 CTX-M-9 resistances, this method includes multiplex real-time PCR, melting temperature profile analysis as well liquid bead microarray assay. Liquid assay is applied only when indistinguishable T m observed. Results The validity assessed isolates. Among the total 580 that were determined by our diagnostic method, 75% them identified PCR with alone, while remaining 25% required both for identification. Compared traditional phenotypic susceptibility test, an overall agreement 81.2% (kappa = 0.614, 95% CI = 0.550–0.679) observed, sensitivity specificity 87.7% 73% respectively. Besides, average test turnaround time 3.9 h, which much shorter comparison more than 24 h tests. Conclusions Having advantages operating comparable high provides efficient tool rapid determination resistances