In vitro determination of normal and neoplastic human brain tissue optical properties using inverse adding-doubling

摘要: To complement a project towards the development of real-time optical biopsy for brain tissue discrimination and surgical resection guidance, properties various tissues were measured in vitro correlated to features within clinical diffuse reflectance spectra vivo. Reflectance transmission samples with single-integrating-sphere spectrometer wavelengths 400-1300 nm converted absorption reduced scattering using an inverse adding-doubling technique. Optical property classified as deriving from white matter, grey matter or glioma according histopathologic diagnosis, mean calculated three categories. Absolute values their relative differences between histopathological groups agreed previously reported results exception that coefficients often overestimated, most likely due biologic variability unaccounted light loss during reflectance/transmission measurement. Absorption classes dominated by haemoglobin below 600 water above 900 generally determined shape corresponding spectra. Reduced spectral shapes followed power curve predicted Rayleigh limit Mie theory. While governed spectra, emission intensities