Promoted activation of matrix metalloproteinase (MMP)-2 in keloid fibroblasts and increased expression of MMP-2 in collagen bundle regions: implications for mechanisms of keloid progression
作者： Risa Imaizumi , Yoshikiyo Akasaka , Naomi Inomata , Emi Okada , Kinji Ito
DOI: 10.1111/J.1365-2559.2009.03287.X
关键词: Scars 、 Matrix metalloproteinase 、 Wound healing 、 Gelatinase A 、 Western blot 、 Extracellular matrix 、 Pathology 、 Keloid 、 Chemistry 、 Fibroblast
摘要: Aims: Keloid is characterized by excessive deposition of collagen, resulting from aberrant extracellular matrix (ECM) production and degradation. The aim was to investigate the role metalloproteinases (MMPs) tissue inhibitor (TIMPs) in pathological wound healing keloids. Methods results: Semiquantitative analysis 60 keloid samples 25 mature scar demonstrated significantly increased expression MMP-2, TIMP-2 TIMP-3 keloids compared with scars. Within regions, MMP-2 higher collagen bundle regions than non-collagen regions. Double immunofluorescence revealed that fibroblasts between bundles exhibited membrane-type 1 MMP (MT1-MMP) co-expression, whereas only evident on edge bundles. Western blot gelatin zymography 13 keloid-derived (KFbs) six normal skin dermal-derived (NFbs) unstimulated KFbs activity NFbs under same conditions. Conclusions: These results together indicate can be promoted cooperation MT1-MMP. This could contribute remodelling invasion into peripheral through degradation ECM.
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