Binding of phencyclidine to the detergent solubilized acetylcholine receptor from Torpedomarmorata
作者： Robert E. Oswald
DOI: 10.1016/0024-3205(83)90120-0
关键词: Torpedo 、 Sodium Cholate 、 Receptor 、 Phencyclidine 、 Acetylcholine receptor 、 Biochemistry 、 Chemistry 、 Cholinergic 、 Binding site 、 Agonist
摘要: Abstract The binding of phencyclidine to the acetylcholine receptor from Torpedo marmorata electroplague was measured following solubilization in sodium cholate followed by exchange for Tween 80. In both membrane-bound and solubilized AChR, addition cholinergic agonists simultaneously with PCP results a 100 1000 fold increase association rate 5 10 dissociation as compared unliganded AChR or equilibrated agonist prior addition. addition, number sites pharmacological properties are not markedly changed soluble receptor. These suggest that can undergo similar conformational transitions 80 form monitor these cases.
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