A Disease Model of Muscle Necrosis Caused by Aeromonas dhakensis Infection in Caenorhabditis elegans.
作者： Po-Lin Chen , Yi-Wei Chen , Chun-Chun Ou , Tzer-Min Lee , Chi-Jung Wu
关键词: Necrosis 、 Microbiology 、 Gene knockdown 、 Caenorhabditis elegans 、 Epidermis (botany) 、 Aeromonas 、 Aeromonas infection 、 Myocyte 、 RNA interference 、 Biology
摘要: A variety of bacterial infections cause muscle necrosis in humans. Caenorhabditis elegans has epidermis and bands that resemble soft tissue structures mammals Here, we developed a model caused by Aeromonas dhakensis infection C. elegans. Our data showed A. infected killed rapidly. Characteristic damage induced was demonstrated vivo. Relative expression levels host necrosis-associated genes, asp-3, asp-4 crt-1 increased significantly after infection. The RNAi sensitive NL2099 rrf-3 (pk1426) worms with knockdown genes prolonged survival Specifically WM118 worms, which restricted only to the cells, conferred significant resistance In contrast, severity toxicity produced hemolysin-deletion mutant is attenuated. another example, shiga-like toxin-producing enterohemorrhagic E. coli (EHEC) known elicit concomitant enteropathogenicty, did not as did. Taken together, these results show induces rapid death elegans, are similar humans, then validate value studying pathogenicity.
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