Deficiency in kwashiorkor serum of factors required for optimal lymphocyte transformation in vitro.

摘要: Blastogenic responses of normal human peripheral blood lymphocytes cultured in media supplemented with serum from children kwashiorkor were, on average, 47.7% those observed when the same cells were presence AB serum. Incorporation radioactive uridine was also diminished indicating that lectin-induced RNA synthesis affected. The effect not due to a cytotoxic action nor could it be attributed saccharides or other inhibitors inducing lectins. Mixing experiments showed inhibitory, but lacked factors present are required for optimal lymphocyte blastogenesis. deficiency these largely rectified by supplementing an ultrafiltrate containing components molecular weights less than 500 Daltons. We conclude nutritional deprivation severity sufficient cause leads low weight growth factors. This lack may contribute immunodeficiency associated disease.