Transcriptome profiling of esophageal squamous cell carcinoma reveals a long noncoding RNA acting as a tumor suppressor.

摘要: // Guifeng Wei 1,2,* , Huaxia Luo Yu Sun Jiagen Li 3 Liqing Tian 1 Liu 1,2 Lihui Jianjun Jie He and Runsheng Chen 1,4 Bioinformatics Laboratory CAS Key of RNA Biology, Institute Biophysics, Chinese Academy Sciences, Beijing, China 2 University Department Thoracic Surgery, Cancer Hospital, Medical Sciences Peking Union College, 4 Research Network Computational RNCB, * These authors have contributed equally to this work Correspondence to: Chen, email: He, Keywords : ESCC, long noncoding RNAs, tumor suppressor, metastasis, Epist Received March 16, 2015 Accepted May 02, Published 19, Abstract Esophageal Squamous Cell Carcinoma (ESCC) is among the most common malignant cancers worldwide. In past, extensive efforts been made characterize involvement protein-coding genes in ESCC tumorigenesis but few for RNAs (lncRNAs). To investigate transcriptome profile functional relevance lncRNAs, we performed an integrative analysis a customized combined lncRNA-mRNA microarray RNA-seq data on ESCCs matched normal tissues. We identified numerous lncRNAs that were differentially expressed between tissues, termed “ESCC-associated (ESCALs)”, which, majority displayed restricted expression pattern. Also, subset ESCALs appeared be associated with patient survival. Gene set enrichment (GSEA) further suggested over half positively- or negatively- metastasis. Among these, novel nuclear-retained lncRNA, named which generally highly esophagus, down-regulated during progression. over-expression knockdown studies suggest inhibits acting as suppressor ESCC. Collectively, our potential suppressing lncRNA provided foundation future identify cancerous therapeutic targeting.