Light-based additive manufacturing of PolyHIPEs: Controlling the surface porosity for 3D cell culture applications

摘要: Abstract Using stereolithography (vat photopolymerisation) to polymerise High Internal Phase Emulsions (PolyHIPEs) is a potent additive manufacturing route produce materials with hierarchical porosity. These multiscale porous have microporosity (1–50 μm) dictated by emulsion templating and macroporosity (100 μm upwards) controlled manufacturing. The interconnected, porosity of these structures particularly desirable in the field bone tissue engineering as it promotes formation allows efficient mass transport. However, due high light-scattering nature HIPEs, achievable feature resolution poor comparison other photocurable polymers, they are prone closed ‘skin layer’ at surface. This study focuses on different methods both improving fabricated from HIPEs via minimising skin formation. inclusion 2-(2H-Benzotriazol-2-yl)-4,6-bis(1-methyl-1-phenylethyl)phenol (commercially UV-234 or Tinuvin®234), UV light-absorber, was found significantly improve PolyHIPE no cytotoxic effects reduce Furthermore, direct non-microporous scaffold same architecture, promoted proliferation MLO-A5 murine osteoblasts permitted superior bone-matrix deposition.