Mutation of tryptophanase gene tnaA in Edwardsiella tarda reduces lipopolysaccharide production, antibiotic resistance and virulence
作者： Yin Han , Chun-Li Yang , Qian Yang , Zizhong Qi , Wenzheng Liu
DOI: 10.1111/J.1758-2229.2011.00269.X
关键词: Indole test 、 Swarming motility 、 Carbenicillin 、 Mutant 、 Microbiology 、 Edwardsiella tarda 、 Virulence 、 Tryptophanase 、 Biology 、 Escherichia coli
摘要: A fish pathogen Edwardsiella tarda LTB-4 produced various indole alkaloids, including indole, 2-(1H-indol-3-yl)ethanol, 4-di(1H-indol-3-yl)methylphenol, tri(1H-indol-3-yl)methane and 2-[2,2-bis(1H-indol-3-yl)]ethylphenylamine. Indole was the most abundant among these alkaloids. E. indoles during its whole growth phase maintained a high level (around 35.5 µM) stationary phase. The relevant tryptophanase (TnaA) gene tnaA cloned from conditionally expressed in Escherichia coli; recombinant TnaA catalysed L-tryptophan to indole. in-frame deletion mutant ΔtnaA constructed through double cross-over allelic exchange by means of suicide vector pRE118; caused some phenotypic changes decreased swarming twitching motility, lipopolysaccharide production multiple antibiotic resistances. Also, subtherapeutic doses chloromycetin, carbenicillin tetracyline could cause decrease bacterial growth, but greatly induce tarda. Most importantly, attenuated virulence zebra increasing LD50 for about 55-fold indicated that involved
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