Dissolved inorganic carbon concentration mechanism in Chlamydomonas moewusii

摘要: Abstract The dissolved inorganic carbon concentrating mechanism(s) of Chlamydomonas moewusii CC 55 was compared with C. reinhardtii strain 137. C. moewusii is similar to respect maximal rates photosynthetic oxygen evolution, CO 2 fixation, respiration, and the ability efficiently concentrate carbon. has a low, but measurable amount external carbonic anhydrase (CA) that not inhibited by acetazolamide (AZ), an inhibitor periplasmic (pCA) in C. reinhardtii. K 0.5 (CO ) for air-grown about 1 μM algal cells accumulated (DIC) level 1 mM 60 s. AZ did inhibit fixation DIC accumulation C. . both species remains constant from pH 6.5 9.5 while (HCO 3 - increased logarithmically, which indicates apparent enters algae. Antiserum prepared against 37 kDa peptide pCA immunoreactive polypeptides 26, 28, 32 kDa activity part mechanism , C  accomplished without AZ-sensitive pCA.