Endothelium-Independent Vasodilatory Effect of Sailuotong (SLT) on Rat Isolated Tail Artery.

摘要: Background Sailuotong (SLT) is a standardized three-herb formulation consisting of extracts Panax ginseng, Ginkgo biloba, and Crocus sativus for the treatment vascular dementia (VaD). Although SLT has been shown to increase cerebral blood flow, direct effects on reactivity have not explored. This study aims examine vasodilatory underlying mechanisms in rat isolated tail artery. Methods Male (250-300 g) Wistar Kyoto (WKY) artery was isometric tension measurement. The influx calcium through cell membrane channels were determined Ca2+-free solution experiments. Results (0.1-5,000 μg/ml) caused concentration-dependent relaxation precontracted by phenylephrine. In contraction experiments, (500, 1,000, 5,000 μg/mL) significantly inhibited phenylephrine (0.001 10 μM)- KCl (10-80 mM)-induced contraction, manner. solution, markedly suppressed Ca2+-induced (0.001-3 mM) vasoconstriction manner both (10 μM) or (80 stimulated arteries. L-type channel blocker nifedipine PE-induced contraction. Furthermore, reduced phenylephrine-induced transient Conclusion induces endothelium-independent mechanisms. SLT-induced vasodilatation appeared be jointly meditated blockages extracellular Ca2+ via receptor-gated voltage-gated inhibition release from sarcoplasmic reticulum.