AML1/Runx1 negatively regulates quiescent hematopoietic stem cells in adult hematopoiesis.
作者： Motoshi Ichikawa , Susumu Goyama , Takashi Asai , Masahito Kawazu , Masahiro Nakagawa
DOI: 10.4049/JIMMUNOL.180.7.4402
关键词: Chromosomal translocation 、 Stem cell 、 Haematopoiesis 、 Homeostasis 、 RUNX1 、 Cell biology 、 Bone marrow 、 Biology 、 Stem cell factor 、 Immunology 、 Side population
摘要: Transcription factor AML1/Runx1, initially isolated from the t(8;21) chromosomal translocation in human leukemia, is essential for development of multilineage hematopoiesis mouse embryos. AML1 negatively regulates number immature hematopoietic cells adult hematopoiesis, whereas it required megakaryocytic maturation and lymphocytic development. However, remains yet to be determined how contributes homeostasis stem (HSCs). To address this issue, we analyzed detail HSC function absence AML1. Notably, Hoechst 33342 side population fraction are increased AML1-deficient bone marrow, which suggests enrichment quiescent HSCs. We also found an increase within marrow using limiting dilution transplantation assays. These results indicate that HSCs regulated by
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