Novel Differential Neuroproteomics Analysis of Traumatic Brain Injury in Rats
作者： Firas H. Kobeissy , Andrew K. Ottens , Zhiqun Zhang , Ming Cheng Liu , Nancy D. Denslow
DOI: 10.1074/MCP.M600157-MCP200
关键词: Traumatic brain injury 、 Proteomics 、 Protein purification 、 Neuroproteomics 、 Medicine 、 Pharmacology 、 Biomarker discovery 、 Hexokinase 、 Transferrin 、 Synaptotagmin 1
摘要: Approximately two million traumatic brain injury (TBI) incidents occur annually in the United States, yet there are no specific therapeutic treatments. The absence of diagnostic endpoints was identified as a significant roadblock to TBI development. To this end, our laboratory has studied mechanisms cellular for biomarker discovery and possible strategies. In study, pooled nao¨ve injured cortical samples (48 h postinjury; rat controlled impact model) were processed analyzed using differential neuroproteomics platform. Protein separation performed combined cation/anion exchange chromatography-PAGE. Differential proteins then trypsinized with reversed-phase LC-MSMS protein identification quantitative confirmation. results included 59 components which 21 decreased 38 increased abundance after TBI. Proteins collapsin response mediator 2 (CRMP-2), glyceraldehyde-3-phosphate dehydrogenase, microtubule-associated MAP2A/2B, hexokinase. Conversely C-reactive protein, transferrin, breakdown products CRMP-2, synaptotagmin, II-spectrin found be elevated changes above mentioned confirmed by immunoblotting. Results from work provide insight into yield putative biochemical markers potentially facilitate patient management monitoring severity, progression, treatment injury. Molecular & Cellular Proteomics 5:1887–1898, 2006.
researchgate.net 
ufl.edu 
sci-hub.se 参考文章(44)
Kevin K.W. Wang, Andrew Ottens, William Haskins, Ming Cheng Liu, Firas Kobeissy, Nancy Denslow, SuShing Chen, Ronald L. Hayes, Proteomics studies of traumatic brain injury. International Review of Neurobiology. ,vol. 61, pp. 215- 240 ,(2004) , 10.1016/S0074-7742(04)61009-9
André Wennersten, Staffan Holmin, Tiit Mathiesen, Characterization of Bax and Bcl-2 in apoptosis after experimental traumatic brain injury in the rat Acta Neuropathologica. ,vol. 105, pp. 281- 288 ,(2003) , 10.1007/S00401-002-0649-Y
L.W. Jenkins, G.W. Peters, C.E. Dixon, X. Zhang, R.S.B. Clark, J.C. Skinner, D.W. Marion, P.D. Adelson, P.M. Kochanek, Conventional and functional proteomics using large format two-dimensional gel electrophoresis 24 hours after controlled cortical impact in postnatal day 17 rats. Journal of Neurotrauma. ,vol. 19, pp. 715- 740 ,(2002) , 10.1089/08977150260139101
Bong Kyung Shin, Proteomics approaches to uncover the repertoire of circulating biomarkers for breast cancer Journal of Mammary Gland Biology and Neoplasia. ,vol. 7, pp. 407- 413 ,(2002) , 10.1023/A:1024038132381
Li Fan, Vincent Jaquet, Peter R. Dodd, Wenbin Chen, Peter A. Wilce, Molecular cloning and characterization of hNP22: a gene up-regulated in human alcoholic brain. Journal of Neurochemistry. ,vol. 76, pp. 1275- 1281 ,(2001) , 10.1046/J.1471-4159.2001.00176.X
Bertil Romner, Tor Ingebrigtsen, Biochemical serum markers for brain damage: a short review with emphasis on clinical utility in mild head injury. Restorative Neurology and Neuroscience. ,vol. 21, pp. 171- 176 ,(2003)
Ming Cheng Liu, Veronica Akle, Wenrong Zheng, Jitendra R. Dave, Frank C. Tortella, Ronald L. Hayes, Kevin K. W. Wang, Comparing calpain- and caspase-3-mediated degradation patterns in traumatic brain injury by differential proteome analysis. Biochemical Journal. ,vol. 394, pp. 715- 725 ,(2006) , 10.1042/BJ20050905
Nancy Denslow, Mary Ellen Michel, Meredith D. Temple, Chung Y. Hsu, Kathryn Saatman, Ronald L. Hayes, Application of proteomics technology to the field of neurotrauma. Journal of Neurotrauma. ,vol. 20, pp. 401- 407 ,(2003) , 10.1089/089771503765355487
Andrew K. Ottens, Firas H. Kobeissy, Erin C. Golden, Zhiqun Zhang, William E. Haskins, Su-Shing Chen, Ronald L. Hayes, Kevin K.W. Wang, Nancy D. Denslow, Neuroproteomics in neurotrauma Mass Spectrometry Reviews. ,vol. 25, pp. 380- 408 ,(2006) , 10.1002/MAS.20073
O Farkas, B Polgar, J Szekeres-Bartho, T Doczi, JT Povlishock, A Büki, Spectrin breakdown products in the cerebrospinal fluid in severe head injury – preliminary observations Acta Neurochirurgica. ,vol. 147, pp. 855- 861 ,(2005) , 10.1007/S00701-005-0559-6