Differential genomic targeting of the transcription factor TAL1 in alternate haematopoietic lineages

摘要: TAL1/SCL is a master regulator of haematopoiesis whose expression promotes opposite outcomes depending on the cell type: differentiation in erythroid lineage or oncogenesis T-cell lineage. Here, we used combination ChIP sequencing and gene profiling to compare function TAL1 normal leukaemic T cells. Analysis genome-wide binding properties these two haematopoietic lineages revealed new insight into mechanism by which transcription factors select their sites alternate lineages. Our study shows limited overlap TAL1-binding profile between types with an unexpected preference for ETS RUNX motifs adjacent E-boxes Furthermore, show that interacts RUNX1 ETS1, are critically required genes modulate differentiation. Thus, our findings highlight critical role cellular environment modulating factor binding, provide inhibits leading