Complete sequence of virulence plasmid pJM1 from the marine fish pathogen Vibrio anguillarum strain 775.

摘要: The virulence plasmid pJM1 enables the fish pathogen Vibrio anguillarum, a gram-negative polarly flagellated comma-shaped rod bacterium, to cause highly fatal hemorrhagic septicemic disease in salmonids and other fishes, leading epizootics throughout world. 65,009-nucleotide sequence, with an overall G+C content of 42.6%, revealed genes open reading frames (ORFs) encoding iron transporters, nonribosomal peptide enzymes, proteins essential for biosynthesis siderophore anguibactin. Of 59 ORFs, approximately 32% were related metabolic functions. confers on V. anguillarum ability take up ferric as complex anguibactin from medium which is chelated by transferrin, ethylenediamine-di(o-hydroxyphenyl-acetic acid), or iron-chelating compounds. fatDCBA-angRT operon well downstream biosynthetic bracketed homologous ISV-A1 ISV-A2 insertion sequences. Other clusters also show element-flanked organization, including ORFs involved 2,3-dihydroxybenzoic acid. Homologues replication partition are identified adjacent this region. no known function represent 30% sequence. sequence elements composite transposon-like structures, corroborated suggest modular composition pJM1, biased towards acquisition modules containing We that there considerable microheterogeneity pJM1-like plasmids virulent strains isolated different geographical sources.