Pursuing mechanisms of extracellular vesicle formation. Effects of sample processing
作者： Darja Božič , Matej Hočevar , Veno Kononenko , Marko Jeran , Urška Štibler
DOI: 10.1016/BS.ABL.2020.09.003
关键词: Biophysics 、 Sample processing 、 Extracellular vesicles 、 Human plasma 、 Extracellular vesicle 、 Vesicle 、 Chemistry 、 Centrifugation
摘要: Abstract Although the role of extracellular vesicles in inter-cell communication is acknowledged, their analytics yet problematic. Poor understanding effects sample processing may be one cornerstones this issue. We investigated centrifugation during vesicle isolation on number, size, content and shape particles isolates. Particles range about 100–500 nm as observed by scanning electron microscope exhibited characteristic shapes without internal structure. Enhanced centrifugal pull resulted increased amount certain types proteins Majority present were highly abundant human plasma proteins, including albumins immunoglobulins. Results are favor hypothesis that isolated reflect substance from which they originate (cells surrounding solution) performed processing; therefore, isolates should viewed upon a dynamic material with transient identity.
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