Association of RAP1 binding sites with stringent control of ribosomal protein gene transcription in Saccharomyces cerevisiae.

摘要: An amino acid limitation in bacteria elicits a global response, called stringent control, that leads to reduced synthesis of rRNA and ribosomal proteins increased expression biosynthetic operons. We have used the antimetabolite 3-amino-1,2,4-triazole cause histidine as means elicit response yeast Saccharomyces cerevisiae. Fusions protein genes RPL16A, CRY1, RPS16A, RPL25 with Escherichia coli lacZ gene were show these is by factor 2 5 during histidine-limited exponential growth this regulation occurs at level transcription. Stringent four was shown be associated nucleotide sequence, known UASrpg (upstream activating sequence for genes), binds transcriptional regulatory RAP1. The RAP1 binding sites also appeared mediate greater observed cells growing exponentially than stationary phase. Although limitation, DNA-binding activity cell extracts unaffected. Yeast strains bearing mutation any one GCN1 GCN4 are defective derepression 10 different pathways under conditions limitation. These Gcn- mutants showed wild-type expression, which suggests separate exist S. cerevisiae repression starvation.