Kinetic isotope effects in the photochemical reaction cycle of ion transporting retinal proteins.
作者： Júliánna Szakács , Melinda Lakatos , Constanta Ganea , György Váró
DOI: 10.1016/J.JPHOTOBIOL.2005.01.001
关键词: Hydrogen–deuterium exchange 、 Bacteriorhodopsin 、 Chemistry 、 Kinetics 、 Kinetic isotope effect 、 Proteorhodopsin 、 Proton 、 Halorhodopsin 、 Deuterium 、 Photochemistry
摘要: Abstract The kinetics of the photochemical reaction cycle bacteriorhodopsin, pharaonis halorhodopsin and proteorhodopsin were determined in H2O D2O at low high pH, to get insight proton dependent steps transport process. While all bacteriorhodopsin photocycle normal pH exhibited a strong isotope effect, uptake step photocycle, measured became independent deuterium exchange, making plausible that this step, concentration, becomes concentration dependent, not mobility dependent. transporting its (9.5) shows marked while (12.2) effect almost totally disappears. It was shown earlier is rise N form. As decreases with rising rate limiting, hiding other components. In case chloride, nitrate conditions strongly affected by exchange. cases first two ions seems normal, absence puzzle. only explanation presence azide transports proton, but negatively charged ion OH−, mass which slightly influenced
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