Separation of oil sands process water organics and inorganics and examination of their acute toxicity using standard in-vitro bioassays.

摘要: Abstract Oil sands process water (OSPW) contains complex components of inorganics and organics. This is the first study that separated OSPW inorganic organic fractions examined their relative acute toxicity when compared with original whole using an in-vitro cell-based bio-indicator system. The separation would be conducive to understanding toxic contribution as well identification treatment fractions. In this research, we demonstrated highest fraction extraction was obtained HLB solid phase 95.4 ± 0.7% dissolved carbon (DOC) 90.0 ± 5.3% naphthenic acid (NA) recovered from OSPW, which were higher than those traditional dichloromethane liquid-liquid (48.8 ± 0.2% DOC 81.0 ± 2.6% NA recovery) or other SPE cartridges tested. We also reported isolation method for by removing 96.1 ± 0.2% in granular activated carbon. difference parameters such pH, alkalinity, conductivity, concentration detected ions between isolated negligible. fraction, reconstituted assessed bioassays RAW 264.7 mouse macrophage cell lines. significant cytotoxicity at 14 mg/L O2-NA affected cellular metabolic activity 10 mg/L O2-NAs. No effect on observed Overall, provides procedure major (i.e., organics inorganics), allows assessment toxicological effects a standard bioassay allow more accurate characterization each OSPW.