Attenuation in the evolution of experimental spinal cord trauma by treatment with melatonin

摘要: Abstract:   Melatonin is the principal secretory product of pineal gland and its role as an immumo-modulator well established. Recent evidence shows that melatonin a scavenger oxyradicals peroxynitrite exerts protective effects in septic shock, hemorrhagic shock inflammation. In present study, we evaluated effect treatment, model spinal cord injury (SCI). SCI was induced by application vascular clips (force 50 g) to dura via four-level T5-T8 laminectomy. rats resulted severe trauma characterized edema, neutrophil infiltration apoptosis (measured terminal deoxynucleotidyl transferase-mediated dUTP nick-end-labeling staining). Infiltration tissue with neutrophils increase myeloperoxidase activity) associated enhanced lipid peroxidation (increased levels malondialdehyde). Immunohistochemical examination demonstrated marked immunoreactivity for nitrotyrosine Poly(ADP-ribose) (PAR) tissue. contrast, degree (a) inflammation (histological score), (b) PAR formation, (c) (d) markedly reduced obtained from treated (50 mg/kg i.p., 30 min before SCI, 30 min, 6 hr, 12 hr 24 hr after SCI). separate set experiment have clearly treatment significantly ameliorated recovery limb function (evaluated motor score). Taken together, our results demonstrate reduces development events trauma.