Cell culture adaptation and propagation of a reovirus-like agent of calf diarrhea from a field outbreak in Nebraska.
作者： A. L. Fernelius , A. E. Ritchie , L. G. Classick , J. O. Norman , C. A. Mebus
DOI: 10.1007/BF01241157
关键词: Microbiology 、 Cytopathic effect 、 Diagnostic aid 、 Virology 、 Specific fluorescence 、 Biology 、 Medical microbiology 、 Diarrhea 、 Virus 、 Outbreak 、 Cell culture
摘要: The Nebraska calf diarrhea viral (NCDV) agent was successfully adapted and propagated in several cell culture systems. Criteria of propagation were: production a cytopathic effect, specific fluorescence, interference with cytopathogenicity another virus, infection neonatal calves, identification the NCDV by electron microscopy (EM) or immuno-electron (I-EM). EM I-EM techniques proved to be valuable diagnostic aids for detecting disease affected calves.
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